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Natures ability to incorporate the thiol group (-S-) into peptide and small molecule natural products (Supplementary Fig. 1a, b) also allows for future thiol addition reactions in the future. For example, cysteine is incorporated into many natural products as cysteine-S-methyltransferase activity 1, 2, 3, 4 . Another example is the Streptomyces aurantiacus cysteine desulfurase CsdA, which introduces cysteine and both the thiol and disulfide groups into the natural products siderophores ferricrocin and enterobactin 6 (Supplementary Fig. 1c). The exogenous thiol group can also serve as a synthetic handle for tethering other molecules to a single-crystal natural product scaffold, in a manner analogous to biotinylation of proteins 20 .
We are pleased to announce that the portable-mestrenova pipeline and collaborative online portal are now available. Using the portable-mestrenova pipeline and online portal, anyone can start annotating metabolites generated from various Natures soil biomes and save them for future use.
The portable-mestrenova pipeline is now ready to assist the scientific community in annotating microbial natural products and is now readily available at https://portablemestrenova.ibcherry.com/ .
We have further improved the portable-mestrenova pipeline by adding four new essential tools: The ability to annotate metabolites from a wide range of taxa from Natures soil biomes, a facile screening tool for identifying and localizing compounds of interest, a facilitated data flow engine to handle the large amounts of data generated, and a sophisticated web interface for easy access to the tools and data. By combining the ability to annotate with a remote a priori knowledge of the biosynthetic potential from the available genome sequences in Natures soil biomes, and the ability to automatically and simultaneously screen the annotated data from various Natures soil biomes by focusing on co-occurrence of chemical scaffolds and taxonomic annotations, the portable-mestrenova pipeline provides an efficient high-throughput method to identify novel microbial natural products.
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